Apples

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More juice from apples

Pectinases and cellulases can be used to enhance the yield of juice from apples and similar fruits

 

Summary
A simple practical protocol, allowing students to investigate how enzymes may be used to enhance the yield of fruit juice from apples and similar fruits.

Educational objectives
Reinforces learning about the stucture of plant tissues. Allows practical, quantitative investigation of factors affecting enzyme activity e.g., temperature and pH. Presents scope for open-ended investigations. Industrial use of enzymes.

Pre-knowledge and skills required by students
Structure of plant cells and tissues, including the nature of the cell wall and pectin between cells. General nature and principles of enzyme activity, including effect of temperature and pH.

Time taken in lesson
50 minutes

Preparation time
30 minutes

Specialist equipment and materials required
Pectinase preparation e.g., Novozymes Pectinex. OPTIONAL: cellulase and amylase preparations

Context where originally used
Very widely used in schools throught the UK since the early 1980s.

Additional information
The NCBE supplies suitable Novozymes enzymes to schools and colleges in the UK. Similar arrangements may exist in other countries.

Author
Dean Madden

Author address
NCBE
University of Reading
2 Earley Gate
Reading
RG6 6AU

Copyright HOLDER(s)
Copyright © Dean Madden, 2006

COPYRIGHT TERMS

attributionsmall Attribution required

noderivativessmall No derivative works

noendorsementsmall No endorsement

sharealikesmall Share and share alike

nocommercialsmall No commercial use without permission

nokitssmall No practical kits without permission

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